Fatal attraction: synthetic musk fragrances compromise multixenobiotic defense systems in mussels

We studied interactions of nitromusk compounds musk ketone and musk xylene and polycyclic musks Galaxolide trade mark (HHCB), Celestolide trade mark (ADBI), Tetralide trade mark (AHTN), and Traseolide trade mark (AITI) with multixenobiotic resistance (mxr) transporters in gill tissue of the marine mussel Mytilus californianus (Conrad, 1837). A competitive substrate transport test with rhodamine B was used to assay modulation of transport activity by musks. All tested musks inhibited the transport activity in the low microm range as indicated by increased accumulation of rhodamine B in the tissue. Compared to known substrates of mxr transporters, the effective concentration range was similar to quinidine and about 100 times higher than verapamil. Musk ketone and musk xylene also inhibited efflux of rhodamine B from gill tissue which was loaded with the dye and subsequently incubated with these compounds. Synthetic musk compounds are persistent environmental pollutants in aquatic environments with a high potential to bioaccumulate. As potent inhibitors of mxr transporters they may also play a role as chemosensitizers that enable toxic mxr substrates to accumulate in cells of aquatic organisms.     

Depth Zonation, Micro habitat, and Morphology of Three Species of Acanthemblemaria (Pisces: Blennioidea) in the Guif of California, Mexico

Abstract. The utilization of the spatial resources of refuge type, size and depth placement is investigated in the three sympatric species of Acanthemblemaria at the cape region of Baja California, Mexico. A. balanorum occupies barnacle testes (Balanus tintinnabulum), A. macrospilus occupies barnacles and mollusk tubes (vermetid gastropods and pholadids), and A. crockeri occupies only mollusk tubes (pholadids). Refuge diameter overlap is higher than overlap in depth. A. crockeri consistently occupies depths below 5m. Competition experiments for barnacle refuges among the three species indicate that A. balanorum is a superior competitor for such refuges. Prior residency of a less superior species changes the outcome of refuge competition in its favor. The morphological specialization of A. balanorum and A. macrospilus is evident in a high correlation between head size and standard length. A. balanorum selects refuges with entrance diameters highly correlated to standard length.     

Vertical habitat partitioning by large calanoid copepods in the oceanic subarctic Pacific during Spring

The copepods Neocalanus plumchrus, N. flemingeri, N. cristatus, and Eucalanus bungii dominate the net zooplankton throughout the subarctic Pacific Ocean. All four species have an extensive seasonal ontogenetic vertical migration, completing most or all of their feeding and somatic growth in spring and early summer. We used stratified tows with MOCNESS and BIONESS instrumented net systems to resolve their upper ocean vertical distributions in May and June of 1984, 1987 and 1988. In each year the feeding copepodite stages of all four species were concentrated above the permanent halocline (roughly from 0 to 150m). However, the four species showed strong vertical species zonation and segregation within this layer. We consistently found a near-surface pair (N. plumchrus and N. flemingeri) and a subsurface pair (N. cristatus and E. bungii). The boundary between these groups shifts vertically, but was sharply defined and was very often coincident with a weak and transient thermocline marking the base of the layer actively mixed by surface wind and wave energy. Diel vertical migration was very limited during our sampling periods.The data suggest that the vertical distribution patterns of the copepods could be set by responses to the local intensity of turbulent mixing in the watercolumn. N. plumchrus and N. flemingeri occupied a stratum characterized by strong turbulence. N. cristatus and E. bungii occupied a stratum that was a local minimum in turbulence profiles. The depth of the boundary between the species pairs was deeper when winds and surface energy inputs were strong. The vertical partition pattern may also be determined by a difference in feeding strategy between the species pairs. N. plumchrus and N. flemingeri may feed on the enhanced protozoan population of the mixed layer, while N. cristatus and E. bungii feed on particle aggregates settling from above.     

Mapping sediment acoustic impedance using remote sensing acoustic techniques in a shallow-water carbonate environment

 Real-time trackline plots of surficial sediment acoustic impedance delineate several sedimentary facies off Garden Key in the Dry Tortugas. The sea floor within a 6×6 km surveyed area consists of carbonate muds (silts), sands and shell, rock, and live corals. The 4-kHz acoustic data supports this finding by providing a pictographic representation of the distribution and structure of several sediment facies types. Plotting the gridded acoustic data with commercial mapping software (Surfer) provides a three-dimensional (3D) perspective of the bottom topography with a color contour map of surficial sediment impedance (upper 0.4 m) draped over the 3D surface.     

A simple shaker table for seismometer calibration

A unique and simple shaker table (shake table or shaking table), designed, constructed, and installed at the Hawaii Institute of Geophysics, has proven to be a valuable aid in testing and calibrating short period seismometers, as well as ocean bottom and ocean sub-bottom seismometer/tilt meter packages. It consists of a platform suspended in a stairwell by a single elastic cord (10 m extended length) driven by GeoSpace HS-10 geophones. Platform motion is monitored by orthogonal reference geophones and tilt meters. The relatively low natural periods of the platform, about 1.9 sec vertical and 6.5 sec horizontal, provide sufficient isolation from local vibrations that calibration can be made near operational amplitudes. Vertical or horizontal driver geophones can be driven by a commercial signal generator or white noise generator, or from magnetic tape output. The table can also be tilted with respect to the drivers to determine tilt tolerances and to calibrate tilt meters. A Hewlett-Packard 3582-A spectrum analyzer, used to analyze both reference and output signals, provides near real-time system cabibration and is an efficient means for investigating parasitic system resonances. The analyzer can also provide a white noise signal source to the driver geophones.Hawaii Institute of Geophysics Contribution 1443.     

Hydrocarbons in bivalve mollusks of Port Valdez, Alaska: Consequences of five years' permitted discharge

Petroleum hydrocarbons were investigated in Mytilus edulis and Macoma balthica at six intertidal sites in Port Valdez, Alaska after three to five years of oil terminal operation. Information about the concentrations of total hydrocarbons, unresolved complex mixture, normal alkanes, isoprenoids, olefins, hopanes and polycyclic aromatic hyrocarbons shows that petroleum accumulation in these bivalve mollusks is greatest at the Valdez municipal boat harbor and a crude oil terminal which has been permitted to discharge 170 kg oil day⁻¹. Accumulation two- to ten-fold less were observed at 3 km from the terminal and boat harbor. The temporal trends in petroleum hydrocarbon concentrations in M. edulis and M. balthica reflect the effects of selective retention and depuration of different hydrocarbon classes as well as variation in hydrocarbon sources. It is not clear whether the petroleum concentrations in Port Valdez bivalves had reached steady state by 1982, the final sampling time reported here.